Recombinant human SIX6 protein
SIX6, also as known as OPTX2 and SIX9, belongs to the SIX/Sine oculis homeobox family contains 1 homeobox DNA-binding domain. The SIX homeobox protein is crucial in embryonic development by providing necessary instructions for the formation of the forebrain and eye development. This protein expressed in the developing and adult retina, hypothalamic and the pituitary regions. Defects in SIX6 are the cause of microphthalmia isolated with cataract type 2. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Recombinant human SIX6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
SIX homeobox 6, MCOPCT2, Six9
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
30.1 kDa (269aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gallardo M.E., et al. (2004) Am. J. Med. Genet. A 129:92-94; ; Lopez-Rios J., et al. (1999) Mech. Dev. 83:155-159
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, 2mM EDTA
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
AAH69413
Uniprot Accession Number
O95475
Species
Human
AA Sequence
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