Recombinant human LIN-7A protein

Lin7A, also as known as MALS1 or VELI1, belongs to the lin-7 family. This protein contains 1 L27 domain and 1 PDZ (DHR) domain. It plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Lin7A is expressed in brain, testis, kidney, placenta and liver. Recombinant human Lin7A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein lin-7 homolog A, LIN-7A, LIN7, MALS-1, TIP-33, VELI1, Mammalian lin-seven protein 1, Tax interaction protein 33, Vertebrate lin-7 homolog 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.4 kDa (256aa) confirmed by MALDI-TOF

Additionnal Information

Lin7A, Protein lin-7 homolog A, LIN-7A, LIN7, MALS-1, TIP-33, VELI1, ATGP1757-10 µg, ATGP1757-20 µg, ATGP1757-50 µg, ATGP1757-100 µg, ATGP1757-250 µg, ATGP1757-500 µg, ATGP1757-1 mg, ATGP1757-10, ATGP1757-20, ATGP1757-50, ATGP1757-100, ATGP1757-250, ATGP1757-500, ATGP1757-1

References & Citations

Shamshiev A., et al. (2000) Immunity 13:255-264 ; ; Winau F., et al. (2004) Nat. Immunol. 5:169-174

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004655

Uniprot Accession Number

O14910

Species

Human

AA Sequence

MLKPSVT SAPTADMATL TVVQPLTLDR DVARAIELLE KLQESGEVPV HKLQSLKKVL QSEFCTAIRE VYQYMHETIT VNGCPEFRAR ATAKATVAAF AASEGHSHPR VVELPKTDEG LGFNVMGGKE QNSPIYISRI IPGGVAERHG GLKRGDQLLS VNGVSVEGEH HEKAVELLKA AKDSVKLVVR YTPKVLEEME ARFEKLRTAR RRQQQQLLIQ QQQQQQQQQT QQNHMS