Recombinant human Josephin 1/JOSD1 protein
JOSD1, also as known as Josephin-1, has low protease activity towards poly-ubiquitin chains (in vitro) . JOSD1 is act as a deubiquitinating enzyme. Recently identified ubiquitin-binding sites in the Josephin domain contribute to ubiquitin chain binding and cleavage. Recombinant human JOSD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Josephin-1, dJ508I15.2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.6 kDa (225aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Weeks S.D., et al. (2011) J. Biol. Chem. 286:4555-4565
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, 2mM EDTA
Scientific Category
Ubiquitination
NCBI Accession Number
NP_055691
Uniprot Accession Number
Q15040
Species
Human
AA Sequence
Frequently Asked Questions
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