Recombinant human RNA polymerase III subunit RPC8/POLR3H protein

POLR3H, also as known as A1665 and RPC8, belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. POLR3H acts as nuclear and cytosolic DNA sensor involved in innate immune response. Recombinant human POLR3H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-directed RNA polymerase III subunit RPC8, RPC22.9, RPC8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.3 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

POLR3H, DNA-directed RNA polymerase III subunit RPC8, RPC22.9, RPC8, ATGP1755-10 µg, ATGP1755-20 µg, ATGP1755-50 µg, ATGP1755-100 µg, ATGP1755-250 µg, ATGP1755-500 µg, ATGP1755-1 mg, ATGP1755-10, ATGP1755-20, ATGP1755-50, ATGP1755-100, ATGP1755-250, ATGP1755-500, ATGP1755-1

References & Citations

Ablasser A., et al. (2009) Nat. Immunol. 10:1065-1072; ; Hu P., et al. (2002) Mol. Cell. Biol. 22:8044-8055

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_612211

Uniprot Accession Number

Q9Y535

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASH TKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN