Recombinant human LIN-28B protein

Lin28B belongs to the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28B is acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Recombinant human Lin28B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein lin-28 homolog B, CSDD2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.5 kDa (273aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

LIN-28B, LIN 28B, LIN28B, Protein lin-28 homolog B, CSDD2, ATGP1754-10 µg, ATGP1754-20 µg, ATGP1754-50 µg, ATGP1754-100 µg, ATGP1754-250 µg, ATGP1754-500 µg, ATGP1754-1 mg, ATGP1754-010, ATGP1754-020, ATGP1754-050, ATGP1754-100, ATGP1754-250, ATGP1754-500, ATGP1754-01M

References & Citations

Heo I., et al. (2008) Mol. Cell 32:276-284; ; Richards M, et al. (2004) Stem Cells 22 (1) : 51-64.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol,2mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_001004317

Uniprot Accession Number

Q6ZN17

Species

Human

AA Sequence

MAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT