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Recombinant human TTC32 protein

TTC32 contains 3 TPR repeats. The tetratricopeptide repeat (TPR) is a structural motif. It consists in a degenerate 34 amino acid sequence motif identified in a wide variety of proteins. TPR is found in tandem arrays of 3-16 motifs, which form scaffolds to mediate protein-protein interactions and often the assembly of multiprotein complexes. TPR-containing proteins include the anaphase-promoting complex (APC) subunits cdc16, cdc23 and cdc27, the NADPH oxidase subunit p67-phox, hsp90-binding immunophilins, transcription factors, the PKR protein kinase inhibitor, the major receptor for peroxisomal matrix protein import PEX5 and mitochondrial import proteins. Recombinant human TTC32 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tetratricopeptide repeat domain 32, TPR repeat protein 32, Tetratricopeptide repeat protein 32

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.7 kDa (174aa) confirmed by MALDI-TOF

Additionnal Information

TTC32, tetratricopeptide repeat domain 32, TPR repeat protein 32, Tetratricopeptide repeat protein 32, ATGP1750-10 µg, ATGP1750-20 µg, ATGP1750-50 µg, ATGP1750-100 µg, ATGP1750-250 µg, ATGP1750-500 µg, ATGP1750-1 mg, ATGP1750-10, ATGP1750-20, ATGP1750-50, ATGP1750-100, ATGP1750-250, ATGP1750-500, ATGP1750-1

References & Citations

The MGC Project Team. (2004) Genome Res. 14:2121-2127

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol,2mM DTT

Scientific Category

Other

NCBI Accession Number

NP_001008238

Uniprot Accession Number

Q5I0X7

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEGQRQE SHATLTLAQA HFNNGEYAEA EALYSAYIRR CACAASSDES PGSKCSPEDL ATAYNNRGQI KYFRVDFYEA MDDYTSAIEV QPNFEVPYYN RGLILYRLGY FDDALEDFKK VLDLNPGFQD ATLSLKQTIL DKEEKQRRNV AKNY
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