Recombinant human LYG2 protein

LYG2, also as known as lysozyme G-like 2 and LYGH, belongs to the glycosyl hydrolase 23 family. Lysozyme plays an important role in human innate immunity by causing bacterial cell lysis. The protein contains a SLT domain, a protein domain present in bacterial lytic transglycosylase (SLT) and in eukaryotic lysozymes (GEWL) . SLT domain catalyzes the cleavage of the beta-1, 4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyglucosamine (GlcNAc) . Recombinant human LYG2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Lysozyme G-like 2, LYGH

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.9 kDa (216aa) confirmed by MALDI-TOF

Additionnal Information

LYG2, lysozyme G-like 2, LYGH, ATGP1749-10 µg, ATGP1749-20 µg, ATGP1749-50 µg, ATGP1749-100 µg, ATGP1749-250 µg, ATGP1749-500 µg, ATGP1749-1 mg, ATGP1749-10, ATGP1749-20, ATGP1749-50, ATGP1749-100, ATGP1749-250, ATGP1749-500, ATGP1749-1

References & Citations

Huang P, et al. (2011) Mol Immunol. 48 (4) :524-31

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol,1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_783862

Uniprot Accession Number

Q86SG7

Species

Human

AA Sequence

SYPFSHS MKPHLHPRLY HGCYGDIMTM KTSGATCDAN SVMNCGIRGS EMFAEMDLRA IKPYQTLIKE VGQRHCVDPA VIAAIISRES HGGSVLQDGW DHRGLKFGLM QLDKQTYHPV GAWDSKEHLS QATGILTERI KAIQKKFPTW SVAQHLKGGL SAFKSGIEAI ATPSDIDNDF VNDIIARAKF YKRQSF