Recombinant human MBD3 protein
Methyl-CpG-binding domain protein 3, also known as MBD3, is a member of the MBD family of transcriptional repressors. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Recombinant human MBD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Methyl-CpG-binding domain protein 3, Methyl CpG binding domain protein 3, Methyl-CpG-binding protein MBD3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
35.2 kDa (314aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nan X., et al. (1998) Nature. 393:386-389; ; Hendrich B., et al. (1999) Mamm Genome. 10: 906-912.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT, 1mM EDTA
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_003917
Uniprot Accession Number
O95983
Species
Human
AA Sequence
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