Recombinant human MBD3 protein

Methyl-CpG-binding domain protein 3, also known as MBD3, is a member of the MBD family of transcriptional repressors. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Recombinant human MBD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Methyl-CpG-binding domain protein 3, Methyl CpG binding domain protein 3, Methyl-CpG-binding protein MBD3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.2 kDa (314aa) confirmed by MALDI-TOF

Additionnal Information

MBD3, Methyl-CpG-binding domain protein 3, Methyl CpG binding domain protein 3, Methyl-CpG-binding protein MBD3, ATGP1747-10 µg, ATGP1747-20 µg, ATGP1747-50 µg, ATGP1747-100 µg, ATGP1747-250 µg, ATGP1747-500 µg, ATGP1747-1 mg, ATGP1747-10, ATGP1747-20, ATGP1747-50, ATGP1747-100, ATGP1747-250, ATGP1747-500, ATGP1747-1

References & Citations

Nan X., et al. (1998) Nature. 393:386-389; ; Hendrich B., et al. (1999) Mamm Genome. 10: 906-912.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT, 1mM EDTA

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003917

Uniprot Accession Number

O95983

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMERKRWE CPALPQGWER EEVPRRSGLS AGHRDVFYYS PSGKKFRSKP QLARYLGGSM DLSTFDFRTG KMLMSKMNKS RQRVRYDSSN QVKGKPDLNT ALPVRQTASI FKQPVTKITN HPSNKVKSDP QKAVDQPRQL FWEKKLSGLN AFDIAEELVK TMDLPKGLQG VGPGCTDETL LSAIASALHT STMPITGQLS AAVEKNPGVW LNTTQPLCKA FMVTDEDIRK QEELVQQVRK RLEEALMADM LAHVEELARD GEAPLDKACA EDDDEEDEEE EEEEPDPDPE MEHV