Recombinant human ECSIT protein

ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF) -6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. Recombinant human ECSIT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Evolutionarily conserved signaling intermediate in Toll pathway mitochondrial, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, SITPEC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.6 kDa (222aa) confirmed by MALDI-TOF

Additionnal Information

ECSIT, Evolutionarily conserved signaling intermediate in Toll pathway mitochondrial, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, SITPEC, ATGP1745-10 µg, ATGP1745-20 µg, ATGP1745-50 µg, ATGP1745-100 µg, ATGP1745-250 µg, ATGP1745-500 µg, ATGP1745-1 mg, ATGP1745-10, ATGP1745-20, ATGP1745-50, ATGP1745-100, ATGP1745-250, ATGP1745-500, ATGP1745-1

References & Citations

Moustakas A., et al. (2003) Genes Dev17:2855-2859; ; Vogel R O., et al. (2007) Genes Dev. 21: 615-624.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_001135937

Uniprot Accession Number

Q9BQ95

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSGTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS