Recombinant human RNA polymerase II subunit RPB11-b2/POLR2J3 protein
DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2) . POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA-directed RNA polymerase II subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
15.5 kDa (138aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Palangat M., et al. (2004) J Mol Biol. 341:429-442; ; uivari A., et al. (2006) Nat Struct Mol Biol. 13: 49-54.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001091084
Uniprot Accession Number
A0A0B4J2F8
Species
Human
AA Sequence
MNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP
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