Recombinant human ATG10 protein

Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Recombinant human ATG10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin-like-conjugating enzyme ATG10, APG10, APG10L, pp12616

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

27.7 kDa (243aa) confirmed by MALDI-TOF

Additionnal Information

ATG10, ubiquitin-like-conjugating enzyme ATG10, APG10, APG10L, pp12616, ATGP1742-10 µg, ATGP1742-20 µg, ATGP1742-50 µg, ATGP1742-100 µg, ATGP1742-250 µg, ATGP1742-500 µg, ATGP1742-1 mg, ATGP1742-10, ATGP1742-20, ATGP1742-50, ATGP1742-100, ATGP1742-250, ATGP1742-500, ATGP1742-1

References & Citations

Nemoto T., et al. (2003) J Bio Chem278:39517-39526; ; Shin J H., et al. (2009) Mol Cells. 27: 37-74.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Ubiquitination

NCBI Accession Number

NP_113670

Uniprot Accession Number

Q9H0Y0

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP