Recombinant human CHMP5 protein

CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs) . Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression. Recombinant human CHMP5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Charged multivesicular body protein 5, Chromatin-modifying protein 5, SNF7 domain-containing protein 2, Vacuolar protein sorting-associated protein 60, Vps60, hVps60, C9orf83, SNF7DC2, HSPC177, CGI-34

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

27 kDa (243aa) confirmed by MALDI-TOF

Additionnal Information

CHMP5, Charged multivesicular body protein 5, C9orf83, CGI-34, HSPC177, PNAS-2, SNF7DC2, Vps60, ATGP1740-10 µg, ATGP1740-20 µg, ATGP1740-50 µg, ATGP1740-100 µg, ATGP1740-250 µg, ATGP1740-500 µg, ATGP1740-1 mg, ATGP1740-10, ATGP1740-20, ATGP1740-50, ATGP1740-100, ATGP1740-250, ATGP1740-500, ATGP1740-1

References & Citations

Howard TL, Stauffer DR, et al. (2001) . J Cell Sci. 2395-404.; ; Ward DM, Vaughn MB, et al. (2005) . J Biol Chem. 18; ;280 (11) :10548-55. Epub 2005 Jan 11.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057494

Uniprot Accession Number

Q9NZZ3

Species

Human

AA Sequence

MNRLFG KAKPKAPPPS LTDCIGTVDS RAESIDKKIS RLDAELVKYK DQIKKMREGP AKNMVKQKAL RVLKQKRMYE QQRDNLAQQS FNMEQANYTI QSLKDTKTTV DAMKLGVKEM KKAYKQVKID QIEDLQDQLE DMMEDANEIQ EALSRSYGTP ELDEDDLEAE LDALGDELLA DEDSSYLDEA ASAPAIPEGV PTDTKNKDGV LVDEFGLPQI PAS