Recombinant human Tryptase beta-2 protein

TPSAB1, also known as tryptase alpha/beta-1, is a tryptase that is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known an endogenous proteinase inhibitor. This protein has been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Tryptase alpha/beta-1, TPS1, TPS2, TPSB1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

30.1 kDa (270aa)

Additionnal Information

Tryptase b2, Tryptase alpha/beta-1, TPS1, TPS2, TPSB1, ATGP1729-10 µg, ATGP1729-20 µg, ATGP1729-50 µg, ATGP1729-100 µg, ATGP1729-250 µg, ATGP1729-500 µg, ATGP1729-1 mg, ATGP1729-10, ATGP1729-20, ATGP1729-50, ATGP1729-100, ATGP1729-250, ATGP1729-500, ATGP1729-1

References & Citations

Tanaka T. et al. (1983) J Biol Chem. 258 (22) :13552-7.; ; Marquardt u. et al. (2002) J Mol Biol. 321 (3) :491-502.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_003285.2

Uniprot Accession Number

Q15661

Species

Human

AA Sequence

IVGGQ EAPRSKWPWQ VSLRVHGPYW MHFCGGSLIH PQWVLTAAHC VGPDVKDLAA LRVQLREQHL YYQDQLLPVS RIIVHPQFYT AQIGADIALL ELEEPVNVSS HVHTVTLPPA SETFPPGMPC WVTGWGDVDN DERLPPPFPL KQVKVPIMEN HICDAKYHLG AYTGDDVRIV RDDMLCAGNT RRDSCQGDSG GPLVCKVNGT WLQAGVVSWG EGCAQPNRPG IYTRVTYYLD WIHHYVPKKP