Recombinant human p21/CIP1/CDKN1A protein

CDKN1A, also known as P21 or WAF1, plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, CDKN1A may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. This protein may be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Recombinant human CDKN1A protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase inhibitor 1A, P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

20.2 kDa (184aa)

Additionnal Information

P21, CIP1, CDKN1A, Cyclin-dependent kinase inhibitor 1A, P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1, ATGP1725-10 µg, ATGP1725-20 µg, ATGP1725-50 µg, ATGP1725-100 µg, ATGP1725-250 µg, ATGP1725-500 µg, ATGP1725-1 mg, ATGP1725-10, ATGP1725-20, ATGP1725-50, ATGP1725-100, ATGP1725-250, ATGP1725-500, ATGP1725-1

References & Citations

EI-Deiry W S., et al. (1994) Cancer Res. 54:1169-1174.; ; Harper J W., et al. (1993) Cell. 75:805-816.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol, 0.1M NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_000380.1

Uniprot Accession Number

P38936

Species

Human

AA Sequence

MSEPAGDVRQ NPCGSKACRR LFGPVDSEQL SRDCDALMAG CIQEARERWN FDFVTETPLE GDFAWERVRG LGLPKLYLPT GPRRGRDELG GGRRPGTSPA LLQGTAEEDH VDLSLSCTLV PRSGEQAEGS PGGPGDSQGR KRRQTSMTDF YHSKRRLIFS KRKP