Recombinant human p21/CIP1/CDKN1A protein
CDKN1A, also known as P21 or WAF1, plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, CDKN1A may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. This protein may be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Recombinant human CDKN1A protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Cyclin-dependent kinase inhibitor 1A, P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
20.2 kDa (184aa)
Additionnal Information
References & Citations
EI-Deiry W S., et al. (1994) Cancer Res. 54:1169-1174.; ; Harper J W., et al. (1993) Cell. 75:805-816.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol, 0.1M NaCl
Scientific Category
Cancer
NCBI Accession Number
NP_000380.1
Uniprot Accession Number
P38936
Species
Human
AA Sequence
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