Recombinant human JAM-C protein

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. JAM3 is localized in the tight junctions between high endothelial cells. unlike other proteins in this family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. JAM3 is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. Recombinant human JAM3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Junctional adhesion molecule C, Junctional adhesion molecule 3, JAM-C, JAMC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26 kDa (234aa) confirmed by MALDI-TOF

Additionnal Information

JAM-C, Junctional adhesion molecule C, Junctional adhesion molecule 3, JAM-C, JAMC, ATGP1723-10 µg, ATGP1723-20 µg, ATGP1723-50 µg, ATGP1723-100 µg, ATGP1723-250 µg, ATGP1723-500 µg, ATGP1723-1 mg, ATGP1723-10, ATGP1723-20, ATGP1723-50, ATGP1723-100, ATGP1723-250, ATGP1723-500, ATGP1723-1

References & Citations

Santoso S., et al. (2002) . J Exp Med. 2; ;196 (5) :679-91.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM EDTA, 5mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_116190

Uniprot Accession Number

Q9BX67

Species

Human

AA Sequence

VNLKSS NRTPVVQEFE SVELSCIITD SQTSDPRIEW KKIQDEQTTY VFFDNKIQGD LAGRAEILGK TSLKIWNVTR RDSALYRCEV VARNDRKEID EIVIELTVQV KPVTPVCRVP KAVPVGKMAT LHCQESEGHP RPHYSWYRND VPLPTDSRAN PRFRNSSFHL NSETGTLVFT AVHKDDSGQY YCIASNDAGS ARCEEQEMEV YDLN