Recombinant human IFT20 protein

IFT20 is part of intraflagellar transport (IFT) particles involved in ciliary process assembly. This protein may play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. IFT20 is expressed in almost all tissues. Recombinant human IFT20 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

IFT20 protein, ADP-ribosylation factor-like 14, ARF7; FLJ22595

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17 kDa (152aa) confirmed by MALDI-TOF

Additionnal Information

IFT20, IFT20 protein, ADP-ribosylation factor-like 14, ARF7; FLJ22595, ATGP1722-10 µg, ATGP1722-20 µg, ATGP1722-50 µg, ATGP1722-100 µg, ATGP1722-250 µg, ATGP1722-500 µg, ATGP1722-1 mg, ATGP1722-10, ATGP1722-20, ATGP1722-50, ATGP1722-100, ATGP1722-250, ATGP1722-500, ATGP1722-1

References & Citations

Jurczyk A., et al. (2004) . J Cell Biol. 30; ;166 (5) :637-43.; ; Baker SA., et al. (2003) . J Biol Chem. 5; ;278 (36) :34211-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

AAH38094

Uniprot Accession Number

Q8IY31

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAKDILGEAG LHFDELNKLR VLDPEVTQQT IELKEECKDF VDKIGQFQKI VGGLIELVDQ LAKEAENEKM KAIGARNLLK SIAKQREAQQ QQLQALIAEK KMQLERYRVE YEALCKVEAE QNEFIDQFIF QK