Recombinant human SH3BGRL2 protein
SH3 domain-binding glutamic acid-rich-like protein 2, also known as SH3BGRL2, is a member of the human SH3BGR family. SH3BGRL2 gene located to chromosome 6q13-15 and its messenger RNA has a large 3' untranslated region containing several AuuuA repeats. SH3BGRL2 codes for a protein of 107 amino acids, which, like SH3BGRL and SH3BGRL3 proteins, is highly homologous to the N-terminal region of the SH3BGR protein and appears to be related to Glutaredoxins and to PKC-interacting cousin of thioredoxin homology domain. Recombinant human SH3BGRL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
14.7 kDa (130aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mazzocco M., et al. (2002) Gene. 291:233-9; ; Egeo A., et al. (1998) Biochem Biophys Res Commun. 247: 302-306.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT
Scientific Category
Redox Proteins
NCBI Accession Number
NP_113657
Uniprot Accession Number
Q9UJC5
Species
Human
AA Sequence
MVIRVFI ASSSGFVAIK KKQQDVVRFL EANKIEFEEV DITMSEEQRQ WMYKNVPPEK KPTQGNPLPP QIFNGDRYCG DYDSFFESKE SNTVFSFLGL KPRLASKAEP
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