Recombinant human MOCS2 protein

MOCS2 is a heterotetrameric synthase composed of two small (MOCS2A) and two large (MOCS2B) subunits. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. MOCS2 functions in the second step of the synthesis of molybdenum cofactor or molybdopterin (MPT) . It catalyzes the formation of MPT from precursor Z by incorporating a dithiolene functional group. Recombinant human MOCS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Molybdenum cofactor synthesis 2, molybdenum cofactor synthesis 2, MCBPE, MOCO1, MOCS2A, MOCS2B, MPTS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25 kDa (224aa) confirmed by MALDI-TOF

Additionnal Information

MOCS2, molybdenum cofactor synthesis 2, molybdenum cofactor synthesis 2, MCBPE, MOCO1, MOCS2A, MOCS2B, MPTS, ATGP1712-10 µg, ATGP1712-20 µg, ATGP1712-50 µg, ATGP1712-100 µg, ATGP1712-250 µg, ATGP1712-500 µg, ATGP1712-1 mg, ATGP1712-10, ATGP1712-20, ATGP1712-50, ATGP1712-100, ATGP1712-250, ATGP1712-500, ATGP1712-1

References & Citations

Leimkuhler S., et al. (2005) Hum Genet. 117: 565-570; ; Reiss J., et al. (1999) Hum Genet . 64: 706-711.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol,1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_004522

Uniprot Accession Number

O96007

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSSL EISSSCFSLE TKLPLSPPLV EDSAFEPSRK DMDEVEEKSK DVINFTAEKL SVDEVSQLVI SPLCGAISLF VGTTRNNFEG KKVISLEYEA YLPMAENEVR KICSDIRQKW PVKHIAVFHR LGLVPVSEAS IIIAVSSAHR AASLEAVSYA IDTLKAKVPI WKKEIYEESS TWKGNKECFW ASNS