Recombinant human TDG protein

Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E. coli

Product Specifications

Product Name Alternative

Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

48.4 kDa (433aa)

Additionnal Information

TDG, ATGP1705-10 µg, ATGP1705-20 µg, ATGP1705-50 µg, ATGP1705-100 µg, ATGP1705-250 µg, ATGP1705-500 µg, ATGP1705-1 mg, ATGP1705-10, ATGP1705-20, ATGP1705-50, ATGP1705-100, ATGP1705-250, ATGP1705-500, ATGP1705-1

References & Citations

Um S., et al. (1998) J Biol Chem. 273:20728-20736.; ; Hardeland u., et al. (2002) EMBO J. 21:1456-1464.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 urea, 10% glycerol, 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

AAH37557

Uniprot Accession Number

Q13569

Species

Human

AA Sequence

MEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA