Recombinant human WBP2 protein

WBP2 (WW domain-binding protein 2) is a steroid hormone receptor coactivator. This protein contains 1 GRAM domain. The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. It binds to the WW domain of Yes kinase-associated protein (YAP1) by its PY motifs. The WW-binding 1 motif mediates interaction with NEDD4. Recombinant human WBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

WW domain binding protein 2, WBP-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.5 kDa (284aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

WBP2, WW domain binding protein 2, WBP-2, ATGP1704-10 µg, ATGP1704-20 µg, ATGP1704-50 µg, ATGP1704-100 µg, ATGP1704-250 µg, ATGP1704-500 µg, ATGP1704-1 mg, ATGP1704-10, ATGP1704-20, ATGP1704-50, ATGP1704-100, ATGP1704-250, ATGP1704-500, ATGP1704-1

References & Citations

Chen H.I., et al. (1997) J. Biol. Chem. 272:17070-17077; ; Pirozzi G., et al. (1997) J. Biol. Chem. 272:14611-14616

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_036610.2

Uniprot Accession Number

Q969T9

Species

Human

AA Sequence

MALNKNH SEGGGVIVNN TESILMSYDH VELTFNDMKN VPEAFKGTKK GTVYLTPYRV IFLSKGKDAM QSFMMPFYLM KDCEIKQPVF GANYIKGTVK AEAGGGWEGS ASYKLTFTAG GAIEFGQRML QVASQASRGE VPSGAYGYSY MPSGAYVYPP PVANGMYPCP PGYPYPPPPP EFYPGPPMMD GAMGYVQPPP PPYPGPMEPP VSGPDVPSTP AAEAKAAEAA ASAYYNPGNP HNVYMPTSQP PPPPYYPPED KKTQ