Recombinant human DKK2 protein

Dickkopf-related protein 2, also known as DKK2, belongs to the dickkopf family. DKK2 antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Recombinant human DKK2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Dickkopf-related protein 2, DKK-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.6 kDa (250aa)

Additionnal Information

Dickkopf related protein 2, Dickkopf-related protein 2, DKK-2, DKK 2, DKK2, ATGP1700-10 µg, ATGP1700-20 µg, ATGP1700-50 µg, ATGP1700-100 µg, ATGP1700-250 µg, ATGP1700-500 µg, ATGP1700-1 mg, ATGP1700-010, ATGP1700-020, ATGP1700-050, ATGP1700-100, ATGP1700-250, ATGP1700-500, ATGP1700-01M

References & Citations

Krupnik V.E., et al. (1999) Gene. 238:301-313; ; Clark H.F., et al. (2003) Genome Res. 13:2265-2270

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Stem cell

NCBI Accession Number

NP_055236

Uniprot Accession Number

Q9UBU2

Species

Human

AA Sequence

KLNSIK SSLGGETPGQ AANRSAGMYQ GLAFGGSKKG KNLGQAYPCS SDKECEVGRY CHSPHQGSSA CMVCRRKKKR CHRDGMCCPS TRCNNGICIP VTESILTPHI PALDGTRHRD RNHGHYSNHD LGWQNLGRPH TKMSHIKGHE GDPCLRSSDC IEGFCCARHF WTKICKPVLH QGEVCTKQRK KGSHGLEIFQ RCDCAKGLSC KVWKDATYSS KARLHVCQKI