Recombinant human MED7 protein
Mediator complex subunit 7, also known as MED7, belongs to the Mediator complex subunit 7 family. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e. g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Recombinant human MED7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Mediator complex subunit 7, ARC34; CRSP33; CRSP9
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
29.6 kDa (256aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zhang X., et al. (2005) Mol. Cell. 19:89-100; ; Jiang Y.W., et al. (1998) Proc. Natl. Acad. Sci. u.S.A. 95:8538-8543
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001094286.1
Uniprot Accession Number
O43513
Species
Human
AA Sequence
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