Recombinant human MED7 protein

Mediator complex subunit 7, also known as MED7, belongs to the Mediator complex subunit 7 family. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e. g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Recombinant human MED7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mediator complex subunit 7, ARC34; CRSP33; CRSP9

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.6 kDa (256aa) confirmed by MALDI-TOF

Additionnal Information

MED7, Mediator complex subunit 7, ARC34; CRSP33; CRSP9, ATGP1698-10 µg, ATGP1698-20 µg, ATGP1698-50 µg, ATGP1698-100 µg, ATGP1698-250 µg, ATGP1698-500 µg, ATGP1698-1 mg, ATGP1698-10, ATGP1698-20, ATGP1698-50, ATGP1698-100, ATGP1698-250, ATGP1698-500, ATGP1698-1

References & Citations

Zhang X., et al. (2005) Mol. Cell. 19:89-100; ; Jiang Y.W., et al. (1998) Proc. Natl. Acad. Sci. u.S.A. 95:8538-8543

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001094286.1

Uniprot Accession Number

O43513

Species

Human

AA Sequence

MGEPQQV SALPPPPMQY IKEYTDENIQ EGLAPKPPPP IKDSYMMFGN QFQCDDLIIR PLESQGIERL HPMQFDHKKE LRKLNMSILI NFLDLLDILI RSPGSIKREE KLEDLKLLFV HVHHLINEYR PHQARETLRV MMEVQKRQRL ETAERFQKHL ERVIEMIQNC LASLPDDLPH SEAGMRVKTE PMDADDSNNC TGQNEHQREN SGHRRDQIIE KDAALCVLID EMNERP