Recombinant human MED7 protein

Mediator complex subunit 7, also known as MED7, belongs to the Mediator complex subunit 7 family. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e. g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Recombinant human MED7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.