Recombinant human MCL1 protein
MCL1, also known as BCL2L3, belongs to the Bcl-2 family. MCL1 is involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. Recombinant human MCL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Specifications
Product Name Alternative
Induced myeloid leukemia cell differentiation protein Mcl-1, BCL2L3, mcl1/EAT, myeloid cell leukemia sequence 1, Bcl-2-like protein 3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
37.2 kDa (347aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bingle C.D., et al. (2000) J. Biol. Chem. 275:22136-22146; ; Bae J., et al. (2000) J. Biol. Chem. 275:25255-25261
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_068779.1
Uniprot Accession Number
Q07820
Species
Human
AA Sequence
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