Recombinant human OAZ1 protein

Ornithine decarboxylase antizyme 1, also known as OAZ1, belongs to the ODC antizyme family. It expression is auto-regulated by polyamine-enhanced translational frameshifting. OAZ1 negatively regulates polyamine synthesis by enhancing the negative feedback loop controlling ornithine decarboxylase (ODC) activity. The OAZ1 encoded by this gene inhibits ornithine decarboxylase and accelerates its degradation. Recombinant human OAZ1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ornithine decarboxylase antizyme 1, AZI, OAZ

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

27.8 kDa (251aa) confirmed by MALDI-TOF

Additionnal Information

OAZ1, Ornithine decarboxylase antizyme 1, AZI, OAZ, ATGP1694-10 µg, ATGP1694-20 µg, ATGP1694-50 µg, ATGP1694-100 µg, ATGP1694-250 µg, ATGP1694-500 µg, ATGP1694-1 mg, ATGP1694-10, ATGP1694-20, ATGP1694-50, ATGP1694-100, ATGP1694-250, ATGP1694-500, ATGP1694-1

References & Citations

Zhang M., et al. (2003) EMBO J. 22: 1488-1496; ; Aoto H., et al. (1997) Genomics. 40: 138-141.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol,1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_004143.1

Uniprot Accession Number

P54368

Species

Human

AA Sequence

MVKSSLQ RILNSHCFAR EKEGDKPSAT IHASRTMPLL SLHSRGGSSS ESSRVSLHCC SNPGPGPRWC SDAPHPPLKI PGGRGNSQRD HNLSANLFYS DDRLNVTEEL TSNDKTRILN VQSRLTDAKR INWRTVLSGG SLYIEIPGGA LPEGSKDSFA VLLEFAEEQL RADHVFICFH KNREDRAALL RTFSFLGFEI VRPGHPLVPK RPDACFMAYT FERESSGEEE E