Recombinant human RhoV protein

Rho-related GTP-binding protein RhoV, also known as RHOV, is a member of the Rho family and small GTPase superfamily. RHOV is a 236 amino acid protein that controls the actin cytoskeleton through activation of the JNK pathway. RHOV functions as a lipid anchor at the cytoplasmic side of the cell membrane and is expressed in placenta, pancreas and fetal brain. Recombinant human RHOV protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Ras homolog family member V, Rho-related GTP-binding protein RhoV, CDC42-like GTPase 2, GTP-binding protein-like 2, Rho GTPase-like protein ARHV, Wnt-1 responsive Cdc42 homolog 2, WRCH-2, ARHV

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

28.6 kDa (259aa)

Additionnal Information

RHOV, Rho-related GTP-binding protein RhoV, ARHV, CHP, WRCH2, ATGP1693-10 µg, ATGP1693-20 µg, ATGP1693-50 µg, ATGP1693-100 µg, ATGP1693-250 µg, ATGP1693-500 µg, ATGP1693-1 mg, ATGP1693-10, ATGP1693-20, ATGP1693-50, ATGP1693-100, ATGP1693-250, ATGP1693-500, ATGP1693-1

References & Citations

Wherlock M., et al. (2002) J Cell Sci. 115:239-240.; ; Boureux A., et al. (2007) Mol Biol Evol. 24:203-216.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea,20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_598378

Uniprot Accession Number

Q96L33

Species

Human

AA Sequence

MPPRELS EAEPPPLRAP TPPPRRRSAP PELGIKCVLV GDGAVGKSSL IVSYTCNGYP ARYRPTALDT FSVQVLVDGA PVRIELWDTA GQEDFDRLRS LCYPDTDVFL ACFSVVQPSS FQNITEKWLP EIRTHNPQAP VLLVGTQADL RDDVNVLIQL DQGGREGPVP QPQAQGLAEK IRACCYLECS ALTQKNLKEV FDSAILSAIE HKARLEKKLN AKGVRTLSRC RWKKFFCFV