Recombinant human UbcH6/UBE2E1 protein
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. uBE2E1 is a member of the E2 ubiquitin-conjugating enzyme family. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Recombinant human uBE2E1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin-conjugating enzyme E2 E1, E2 ubiquitin-conjugating enzyme E1, UbcH6, Ubiquitin carrier protein E1, Ubiquitin-protein ligase E1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.8 kDa (216aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nuber u, et al. (1996) . J Biol Chem. 271 (5) : 2795-800. ; ; Takeuchi T, et al. (2005) . J. Biochem. 138 (6) : 711-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol
Scientific Category
Ubiquitination
NCBI Accession Number
NP_003332
Uniprot Accession Number
P51965
Species
Human
AA Sequence
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