Recombinant human CREG/CREG1 protein

CREG1 both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. This protein antagonizes transcriptional activation and cellular transformation by E1A. It shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. CREG1 may contribute to the transcriptional control of cell growth and differentiation. Recombinant human CREG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cellular repressor of E1A-stimulated genes 1, CREG

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.6 kDa (213aa) confirmed by MALDI-TOF

Additionnal Information

CREG, Cellular repressor of E1A-stimulated genes 1, CREG, ATGP1689-10 µg, ATGP1689-20 µg, ATGP1689-50 µg, ATGP1689-100 µg, ATGP1689-250 µg, ATGP1689-500 µg, ATGP1689-1 mg, ATGP1689-10, ATGP1689-20, ATGP1689-50, ATGP1689-100, ATGP1689-250, ATGP1689-500, ATGP1689-1

References & Citations

Wang, N., et al. (2011) Atherosclerosis 218 (2), 543-551; ; Han, Y., et al. (2011) J. Mol. Cell. Cardiol. 50 (4), 723-730

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_003842

Uniprot Accession Number

O75629

Species

Human

AA Sequence

RGGRDH GDWDEASRLP PLPPREDAAR VARFVTHVSD WGALATISTL EAVRGRPFAD VLSLSDGPPG AGSGVPYFYL SPLQLSVSNL QENPYATLTM TLAQTNFCKK HGFDPQSPLC VHIMLSGTVT KVNETEMDIA KHSLFIRHPE MKTWPSSHNW FFAKLNITNI WVLDYFGGPK IVTPEEYYNV TVQ