Recombinant human NEU-1/Sialidase-1 protein
NEu1 is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A. Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red -myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. Recombinant human NEu1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Sialidase 1 (lysosomal sialidase), NANH, NEu, SIAL1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
42.9 kDa (393aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Abdulkhalek, S., et al. (2011) J. Biol. Chem. 286 (42), 36532-36549
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_000425.1
Uniprot Accession Number
Q99519
Species
Human
AA Sequence
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