Recombinant human NHEJ1 protein
Non-homologous end-joining factor 1, also known as NHEJ1, belongs to the XLF family. NHEJ1 was originally discovered as the protein mutated in five patients with growth retardation, microcephaly, and immunodeficiency. The protein is required for the non-homologous end joining (NHEJ) pathway of DNA repair. Patients with NHEJ1 mutations also have immunodeficiency due to a defect in V (D) J recombination, which utilizes NHEJ to promote immune system diversity. XLF interacts with DNA ligase IV and XRCC4 and is thought to be involved in the ligation step of NHEJ. Recombinant human NHEJ1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Non-homologous end-joining factor 1, XLF
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
27.8 kDa (247aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Buck D., et al. (2006) Cell. 124:287-299; ; Ahnesorg P., et al. (2006) Cell. 124:301-313
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_079058
Uniprot Accession Number
Q9H9Q4
Species
Human
AA Sequence
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