Recombinant human UbcH10/UBE2C protein
Ubiquitin-conjugating enzyme E2 C, also known as uBE2C, is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin-conjugating enzyme E2 C, E2 ubiquitin-conjugating enzyme C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
22.1 kDa (202aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Okamoto Y., et al. (2003) Cancer Res. 63:4167-4173; ; Wagner K.W., et al. (2004) Oncogene. 23:6621-6629
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_008950
Uniprot Accession Number
O00762
Species
Human
AA Sequence
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