Recombinant human UbcH10/UBE2C protein

Ubiquitin-conjugating enzyme E2 C, also known as uBE2C, is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin-conjugating enzyme E2 C, E2 ubiquitin-conjugating enzyme C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.1 kDa (202aa) confirmed by MALDI-TOF

Additionnal Information

UbcH10, UBE2C, ubiquitin-conjugating enzyme E2 C, dJ447F3.2; uBCH10, ATGP1681-10 µg, ATGP1681-20 µg, ATGP1681-50 µg, ATGP1681-100 µg, ATGP1681-250 µg, ATGP1681-500 µg, ATGP1681-1 mg, ATGP1681-10, ATGP1681-20, ATGP1681-50, ATGP1681-100, ATGP1681-250, ATGP1681-500, ATGP1681-1

References & Citations

Okamoto Y., et al. (2003) Cancer Res. 63:4167-4173; ; Wagner K.W., et al. (2004) Oncogene. 23:6621-6629

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Ubiquitination

NCBI Accession Number

NP_008950

Uniprot Accession Number

O00762

Species

Human

AA Sequence

MASQNRD PAATSVAAAR KGAEPSGGAA RGPVGKRLQQ ELMTLMMSGD KGISAFPESD NLFKWVGTIH GAAGTVYEDL RYKLSLEFPS GYPYNAPTVK FLTPCYHPNV DTQGNICLDI LKEKWSALYD VRTILLSIQS LLGEPNIDSP LNTHAAELWK NPTAFKKYLQ ETYSKQVTSQ EP