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Recombinant human NPM2 protein

NPM2, also known as nucleophosmin/nucleoplasmin 2, belongs to the nucleoplasmin family. This protein is core histones chaperone involved in chromatin reprogramming, especially during fertilization and early embryonic development. It probably involved in sperm DNA decondensation during fertilization. Recombinant human NPM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nucleophosmin/ nucleoplasmin 2, NPM-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

26.6 kDa (237aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

NPM2, nucleophosmin/ nucleoplasmin 2, NPM-2, ATGP1676-10 µg, ATGP1676-20 µg, ATGP1676-50 µg, ATGP1676-100 µg, ATGP1676-250 µg, ATGP1676-500 µg, ATGP1676-1 mg, ATGP1676-10, ATGP1676-20, ATGP1676-50, ATGP1676-100, ATGP1676-250, ATGP1676-500, ATGP1676-1

References & Citations

Platonova O., et al. (2011) Biochemistry 50:8078-8089

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT

Scientific Category

Development

NCBI Accession Number

NP_877724

Uniprot Accession Number

Q86SE8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMNLSSAS STEEKAVTTV LWGCELSQER RTWTFRPQLE GKQSCRLLLH TICLGEKAKE EMHRVEILPP ANQEDKKMQP VTIASLQASV LPMVSMVGVQ LSPPVTFQLR AGSGPVFLSG QERYEASDLT WEEEEEEEGE EEEEEEEDDE DEDADISLEE QSPVKQVKRL VPQKQASVAK KKKLEKEEEE IRASVRDKSP VKKAKATARA KKPGFKK

Frequently Asked Questions

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