Recombinant human NPM2 protein
NPM2, also known as nucleophosmin/nucleoplasmin 2, belongs to the nucleoplasmin family. This protein is core histones chaperone involved in chromatin reprogramming, especially during fertilization and early embryonic development. It probably involved in sperm DNA decondensation during fertilization. Recombinant human NPM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Nucleophosmin/ nucleoplasmin 2, NPM-2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
26.6 kDa (237aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Platonova O., et al. (2011) Biochemistry 50:8078-8089
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT
Scientific Category
Development
NCBI Accession Number
NP_877724
Uniprot Accession Number
Q86SE8
Species
Human
AA Sequence
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