Recombinant human PAFAH1B3 protein
PAFAH1B3 protein belongs to the 'GDSL' lipolytic enzyme family. Acetylhydrolase is catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. PAFAH1B3 is a subunit of the platelet-activating factor cetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex plays an important role during the development of brain. Recombinant human PAFAH1B3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Platelet-activating factor acetylhydrolase IB subunit gamma, PAFAHG, PAF-AH1b alpha 1 subunit
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
28.2 kDa (254aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Moro F, et al. (1998) Genomics 51 (1) : 157-9; ; TW, Li J, et al. (2001) Protein Eng. 14 (7) : 513-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_001139411
Uniprot Accession Number
Q15102
Species
Human
AA Sequence
Frequently Asked Questions
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