Recombinant human TBCC protein
TBCC (tubulin folding cofactor C) belongs to the TBCC family. It is plays a role in the regulation of centrosome and Golgi apparatus positioning, with consequences on cell shape and cell migration. Cofactor C is one of four proteins (cofactors A, D, E and C) involved in the pathway leading to correctly folded b-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing b-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/b-tubulin complex; interaction with cofactor C then causes the release of b-tubulin polypeptides. Recombinant human TBCC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Tubulin-specific chaperone C, CFC, tubulin folding cofactor C
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
41.7 kDa (369aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Goncalves J., et al. (2010) EMBO Rep. 11:194-200; ; Lewis SA, et al. (1996) . J. Cell Biol. 132 (1-2) : 1-4.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_003183
Uniprot Accession Number
Q15814
Species
Human
AA Sequence
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