Recombinant human SYCE3 protein
SYCE3 (synaptonemal complex central element protein 3) is major component of the transverse central element of synaptonemal complexes (SCS) . The synaptonemal complex is a protein structure that forms between homologous chromosomes (two pairs of sister chromatids) during meiosis and that is thought to mediate chromosome pairing, synapsis, and recombination (crossing-over) . This protein required for chromosome loading of the central element-specific SCS proteins, and for initiating synapsis between homologous chromosomes. Recombinant human SYCE3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
12.8 kDa (108aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Schramm S, et al. (2011) PLoS Genet, May; ;7 (5) :e1002088
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT
Scientific Category
Cell Cycle
NCBI Accession Number
NP_001116697
Uniprot Accession Number
A1L190
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MDDADPEERN YDNMLKMLSD LNKDLEKLLE EMEKISVQAT WMAYDMVVMR TNPTLAESMR RLEDAFVNCK EEMEKNWQEL LHETKQRL
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