Recombinant human ARC/NOL3 protein

Nucleolar protein 3 (apoptosis repressor with CARD domain), also known as NOL3, is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. NOL3 mRNA and protein levels are induced by neuronal activity, which is necessary to stimulate neuroplasticity, indicating a potential role for NOL3 in activity-dependent changes in dendrite function. NOL3 has been shown to localize to the cytoskeleton of neuronal cells and appears to colocalize with F-Actin, although it may associate with an actin-associated protein rather than directly with F-Actin. Recombinant human NOL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nucleolar protein 3, ARC, MYP, NOP, NOP30

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25 kDa (231aa) confirmed by MALDI-TOF

Additionnal Information

NOL3, Nucleolar protein 3, ARC, MYP, NOP, NOP30, ATGP1671-10 µg, ATGP1671-20 µg, ATGP1671-50 µg, ATGP1671-100 µg, ATGP1671-250 µg, ATGP1671-500 µg, ATGP1671-1 mg, ATGP1671-10, ATGP1671-20, ATGP1671-50, ATGP1671-100, ATGP1671-250, ATGP1671-500, ATGP1671-1

References & Citations

Lau L F., et al. (1991) Elseveier Science Pub. 257-293; ; Greenberg M E., et al. (1986) Science. 234: 80-83.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT, 1mM EDTA

Scientific Category

Apoptosis

NCBI Accession Number

NP_001171987

Uniprot Accession Number

O60936

Species

Human

AA Sequence

MGNAQER PSETIDRERK RLVETLQADS GLLLDALLAR GVLTGPEYEA LDALPDAERR VRRLLLLVQG KGEAACQELL RCAQRTAGAP DPAWDWQHVG PGYRDRSYDP PCPGHWTPEA PGSGTTCPGL PRASDPDEAG GPEGSEAVQS GTPEEPEPEL EAEASKEAEP EPEPEPELEP EAEAEPEPEL EPEPDPEPEP DFEERDESED S