Recombinant human ARC/NOL3 protein
Nucleolar protein 3 (apoptosis repressor with CARD domain), also known as NOL3, is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. NOL3 mRNA and protein levels are induced by neuronal activity, which is necessary to stimulate neuroplasticity, indicating a potential role for NOL3 in activity-dependent changes in dendrite function. NOL3 has been shown to localize to the cytoskeleton of neuronal cells and appears to colocalize with F-Actin, although it may associate with an actin-associated protein rather than directly with F-Actin. Recombinant human NOL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Nucleolar protein 3, ARC, MYP, NOP, NOP30
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25 kDa (231aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lau L F., et al. (1991) Elseveier Science Pub. 257-293; ; Greenberg M E., et al. (1986) Science. 234: 80-83.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT, 1mM EDTA
Scientific Category
Apoptosis
NCBI Accession Number
NP_001171987
Uniprot Accession Number
O60936
Species
Human
AA Sequence
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