Recombinant human THG1L protein

Probable tRNA (His) guanylyltransferase, also known as THG1L, belongs to the tRNA (His) gyanylytransferase family. Localized to the cytoplasm, THG1L is also found near the nuclear membrane and is expressed in many tissues, including liver and lung. upon DNA damage, THG1L is phosphorylated, most likely by ATM or ATR. It is expressed in a cell cycle-dependent manner and may be involved in cell cycle progression and cell proliferation. Recombinant human THG1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Probable tRNA (His) guanylyltransferase, ICF45

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34 kDa (292aa) confirmed by MALDI-TOF

Additionnal Information

THG1L, Probable tRNA (His) guanylyltransferase, ICF45, ATGP1668-10 µg, ATGP1668-20 µg, ATGP1668-50 µg, ATGP1668-100 µg, ATGP1668-250 µg, ATGP1668-500 µg, ATGP1668-1 mg, ATGP1668-10, ATGP1668-20, ATGP1668-50, ATGP1668-100, ATGP1668-250, ATGP1668-500, ATGP1668-1

References & Citations

Guo D., et al. (2004) J Biol Chem. 279 (51) : 53498-505.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT

Scientific Category

Cell Cycle

NCBI Accession Number

NP_060342

Uniprot Accession Number

Q9NWX6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAKSKFE YVRDFEADDT CLAHCWVVVR LDGRNFHRFA EKHNFAKPND SRALQLMTKC AQTVMEELED IVIAYGQSDE YSFVFKRKTN WFKRRASKFM THVASQFASS YVFYWRDYFE DQPLLYPPGF DGRVVVYPSN QTLKDYLSWR QADCHINNLY NTVFWALIQQ SGLTPVQAQG RLQGTLAADK NEILFSEFNI NYNNELPMYR KGTVLIWQKV DEVMTKEIKL PTEMEGKKMA VTRTRTKPVP LHCDIIGDAF WKEHPEILDE DS