Recombinant human C4BPB protein

C4BPB is a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain of this protein assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. Recombinant human C4BPB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Complement component 4 binding protein beta, C4BP, C4b-binding protein beta chain

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29kDa (260aa) confirmed by MALDI-TOF

Additionnal Information

C4BPB, ATGP1664-10 µg, ATGP1664-20 µg, ATGP1664-50 µg, ATGP1664-100 µg, ATGP1664-250 µg, ATGP1664-500 µg, ATGP1664-1 mg, ATGP1664-10, ATGP1664-20, ATGP1664-50, ATGP1664-100, ATGP1664-250, ATGP1664-500, ATGP1664-1

References & Citations

Carlsson, S.et al. (2010) J. Biol. Chem. 285 (42), 32038-32046; ; Buil, A., et al. (2010) Blood 115 (23), 4644-4650

Other References

Sugimoto M, et al. Increased expression of Protein S in eyes with diabetic retinopathy and diabetic macular edema. (Sci Rep. 2021) {https://pubmed.ncbi.nlm.nih.gov/34001977/}; ; Urawa M, et al. Protein S is protective in pulmonary fibrosis. (J Thromb Haemost. 2016) {https://pubmed.ncbi.nlm.nih.gov/27172994/}; ; Yasuma T, et al. Amelioration of Diabetes by Protein S. (Diabetes. 2016) {https://pubmed.ncbi.nlm.nih.gov/27207541/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Scientific Category

Immunology

NCBI Accession Number

NP_000707

Uniprot Accession Number

P20851

Species

Human

AA Sequence

SDAEH CPELPPVDNS IFVAKEVEGQ ILGTYVCIKG YHLVGKKTLF CNASKEWDNT TTECRLGHCP DPVLVNGEFS SSGPVNVSDK ITFMCNDHYI LKGSNRSQCL EDHTWAPPFP ICKSRDCDPP GNPVHGYFEG NNFTLGSTIS YYCEDRYYLV GVQEQQCVDG EWSSALPVCK LIQEAPKPEC EKALLAFQES KNLCEAMENF MQQLKESGMT MEELKYSLEL KKAELKAKLL