Recombinant human OLA1 protein

OLA1, also known as obg-like ATPase 1, functions as a negative regulator of the cellular antioxidant response independent of transcriptional processes. OLA1 suppresses the antioxidant response through nontranscriptional mechanisms. Recombinant human OLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Obg-like ATPase 1, DOC45, GBP45, GTBP9, GTPBP9, PTD004

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

47.3 kDa (420aa) confirmed by MALDI-TOF

Additionnal Information

OLA1, Obg-like ATPase 1, DOC45, GBP45, GTBP9, GTPBP9, PTD004, ATGP1660-10 µg, ATGP1660-20 µg, ATGP1660-50 µg, ATGP1660-100 µg, ATGP1660-250 µg, ATGP1660-500 µg, ATGP1660-1 mg, ATGP1660-10, ATGP1660-20, ATGP1660-50, ATGP1660-100, ATGP1660-250, ATGP1660-500, ATGP1660-1

References & Citations

Choudhary C., et al. (2009) Science. 325:834-840; ; Koller-Eichhorn R., et al. (2007) J. Biol. Chem. 282:19928-19937

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_037473

Uniprot Accession Number

Q9NTK5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMPPKKG GDGIKPPPII GRFGTSLKIG IVGLPNVGKS TFFNVLTNSQ ASAENFPFCT IDPNESRVPV PDERFDFLCQ YHKPASKIPA FLNVVDIAGL VKGAHNGQGL GNAFLSHISA CDGIFHLTRA FEDDDITHVE GSVDPIRDIE IIHEELQLKD EEMIGPIIDK LEKVAVRGGD KKLKPEYDIM CKVKSWVIDQ KKPVRFYHDW NDKEIEVLNK HLFLTSKPMV YLVNLSEKDY IRKKNKWLIK IKEWVDKYDP GALVIPFSGA LELKLQELSA EERQKYLEAN MTQSALPKII KAGFAALQLE YFFTAGPDEV RAWTIRKGTK APQAAGKIHT DFEKGFIMAE VMKYEDFKEE GSENAVKAAG KYRQQGRNYI VEDGDIIFFK FNTPQQPKKK