Recombinant human CSTF1 protein

CSTF1, also known as cleavage stimulation factor subunit 1, is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' uTR, but encoding the same protein, have been found for this gene. Recombinant human CSTF1 protein, fused to His-tag at C-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cleavage stimulation factor subunit 1, CstF-50, CstFp50

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

49.4 kDa (439aa)

Additionnal Information

CSTF1, Cleavage stimulation factor subunit 1, CstF-50, CstFp50, ATGP1659-10 µg, ATGP1659-20 µg, ATGP1659-50 µg, ATGP1659-100 µg, ATGP1659-250 µg, ATGP1659-500 µg, ATGP1659-1 mg, ATGP1659-10, ATGP1659-20, ATGP1659-50, ATGP1659-100, ATGP1659-250, ATGP1659-500, ATGP1659-1

References & Citations

Rush J., et al. (2005) Nat. Biotechnol. 23:94-101; ; Takagaki Y., et al. (2000) Mol. Cell. Biol. 20:1515-1525

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 2M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001028693

Uniprot Accession Number

Q05048

Species

Human

AA Sequence

MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DVEHHHHHH