Recombinant human Ephrin-B3 protein

EFNB3, also known as ephrin-B3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. It binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. Recombinant human EFNB3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Ephrin-B3, EFL6, EPLG8, LERK8, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.6 kDa (224aa)

Additionnal Information

Ephrin-B3, ATGP1658-10 µg, ATGP1658-20 µg, ATGP1658-50 µg, ATGP1658-100 µg, ATGP1658-250 µg, ATGP1658-500 µg, ATGP1658-1 mg, ATGP1658-10, ATGP1658-20, ATGP1658-50, ATGP1658-100, ATGP1658-250, ATGP1658-500, ATGP1658-1

References & Citations

Negrete O.A., et al. (2006) PLoS Pathog. 2:78-86; ; Brueckner K., et al. (1999) Neuron. 22:511-524

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 2M urea

Scientific Category

Neuroscience

NCBI Accession Number

NP_001397

Uniprot Accession Number

Q15768

Species

Human

AA Sequence

LSLEP VYWNSANKRF QAEGGYVLYP QIGDRLDLLC PRARPPGPHS SPNYEFYKLY LVGGAQGRRC EAPPAPNLLL TCDRPDLDLR FTIKFQEYSP NLWGHEFRSH HDYYIIATSD GTREGLESLQ GGVCLTRGMK VLLRVGQSPR GGAVPRKPVS EMPMERDRGA AHSLEPGKEN LPGDPTSNAT SRGAEGPLPP PSMP