Recombinant human RBBP4 protein

Retinoblastoma binding protein 4, also known as RBBP4, is a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. RBBP4 is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. Recombinant human RBBP4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Retinoblastoma binding protein 4, NuRF55, RBAP48

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

50.2 kDa (449aa)

Additionnal Information

RBBP4, Retinoblastoma binding protein 4, NuRF55, RBAP48, ATGP1656-10 µg, ATGP1656-20 µg, ATGP1656-50 µg, ATGP1656-100 µg, ATGP1656-250 µg, ATGP1656-500 µg, ATGP1656-1 mg, ATGP1656-10, ATGP1656-20, ATGP1656-50, ATGP1656-100, ATGP1656-250, ATGP1656-500, ATGP1656-1

References & Citations

Qian YW., et al. (1993) Nature. 364 (6438) : 648-52; ; Nicolas E., et al. (2001) Nucleic Acids Res. 29 (15) : 3131-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005601

Uniprot Accession Number

Q09028

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMADKEA AFDDAVEERV INEEYKIWKK NTPFLYDLVM THALEWPSLT AQWLPDVTRP EGKDFSIHRL VLGTHTSDEQ NHLVIASVQL PNDDAQFDAS HYDSEKGEFG GFGSVSGKIE IEIKINHEGE VNRARYMPQN PCIIATKTPS SDVLVFDYTK HPSKPDPSGE CNPDLRLRGH QKEGYGLSWN PNLSGHLLSA SDDHTICLWD ISAVPKEGKV VDAKTIFTGH TAVVEDVSWH LLHESLFGSV ADDQKLMIWD TRSNNTSKPS HSVDAHTAEV NCLSFNPYSE FILATGSADK TVALWDLRNL KLKLHSFESH KDEIFQVQWS PHNETILASS GTDRRLNVWD LSKIGEEQSP EDAEDGPPEL LFIHGGHTAK ISDFSWNPNE PWVICSVSED NIMQVWQMAE NIYNDEDPEG SVDPEGQGS