Recombinant human ITPK1 protein

Inositol-tetrakisphosphate 1-kinase, also known as ITPK1, is a 414 amino acid monomer that belongs to the ITPK1 family and exists as two alternatively spliced isoforms. ITPK1 is found at highest levels in brain, followed by heart, skeletal muscle, kidney, pancreas, liver, placenta and lung. ITPK1 contains one ATP-grasp domain and has been found to phosphorylate various inositol polyphosphates and modify TNFalpha induced apoptosis. Recombinant human ITPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Inositol-tetrakisphosphate 1-kinase, ITRPK1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

48.1 kDa (438aa) confirmed by MALDI-TOF

Additionnal Information

ITPK1, Inositol-tetrakisphosphate 1-kinase, ITRPK1, ATGP1655-10 µg, ATGP1655-20 µg, ATGP1655-50 µg, ATGP1655-100 µg, ATGP1655-250 µg, ATGP1655-500 µg, ATGP1655-1 mg, ATGP1655-10, ATGP1655-20, ATGP1655-50, ATGP1655-100, ATGP1655-250, ATGP1655-500, ATGP1655-1

References & Citations

Sun Y., et al. (2002) J Biol Chem. 277: 45759-45764; ; Chamberlain P P., et al. (2007) J Biol Chem. 282: 28117-28125.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_055031.2

Uniprot Accession Number

Q13572

Species

Human

AA Sequence

MQTFLK GKRVGYWLSE KKIKKLNFQA FAELCRKRGM EVVQLNLSRP IEEQGPLDVI IHKLTDVILE ADQNDSQSLE LVHRFQEYID AHPETIVLDP LPAIRTLLDR SKSYELIRKI EAYMEDDRIC SPPFMELTSL CGDDTMRLLE KNGLTFPFIC KTRVAHGTNS HEMAIVFNQE GLNAIQPPCV VQNFINHNAV LYKVFVVGES YTVVQRPSLK NFSAGTSDRE SIFFNSHNVS KPESSSVLTE LDKIEGVFER PSDEVIRELS RALRQALGVS LFGIDIIINN QTGQHAVIDI NAFPGYEGVS EFFTDLLNHI ATVLQGQSTA MAATGDVALL RHSKLLAEPA GGLVGERTCS ASPGCCGSMM GQDAPWKAEA DAGGTAKLPH QRLGCNAGVS PSFQQHCVAS LATKASSQ