Recombinant mouse S100A3 protein
S100A3, also known as Protein S-100E and S100 calcium-binding protein A3, belongs to the S-100 family of proteins containing 2 EF-hand calcium-binding motifs. The S100 proteins act as multifactional signaling factors that are involved in the regulation of diverse cellular processes such as cell cycle progression and differentiation. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. This protein has the highest content of cysteines of all S100 proteins, has a high affinity for Zinc, and is highly expressed in human hair cuticle. Recombinant mouse S100A3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
S100 calcium binding protein A3, S100E
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
14.3 kDa (125aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Streicher WW, et al. (2010) Biophys Chem. 151 (3) :181-6.; ; Yao R, et al. (2007) Anticancer Res. 27 (5A) :3051-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 150 mM NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_035440
Uniprot Accession Number
P62818
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMTRPLE QAVAAIVCTF QEYAGRCGDK YKICQSELKE LLQKELPTWT PSEFRECDYN KFMSVLDTNK DCEVDFGEYV RSLASLCLYC HEYFKECPPE PPCPQ
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